BBEMG - Belgian BioElectroMagnetics Group

Belgian BioElectroMagnetics Group

EMF and cell differentiation (in vitro study): Reports and publications

EMF and cell differentiation (in vitro study): Reports and publications

2014-2016 activity report

M. Hinsenkamp, J.-F. Collard

We observed a biological response after low frequency and low amplitude pulsed electric or electromagnetic fields exposure on different tissues (bones of mouse embryos in vitro [Hinsenkamp et al., 1982], bones of chicken embryos in vivo [Rooze et al., 1985], bones of quails embryo in vivo [Hinsenkamp et al., 1985b], human epidermal explant culture in vitro [Hinsenkamp et al., 1997; Collard et al., 2011] and in clinical applications [Hinsenkamp et al., 1984, 1985, 1993]) but the precise reaction at the cells level is still not known precisely. In our different models, the biological response to the low frequency electromagnetic fields for each model is an acceleration of the differentiation at the expense of the proliferation.

Considering the very coherent response of our different models we tried to identify the mechanism involved at the cell level through an analysis of the biological pathways and the potential changes in the cellular functions with data obtain by the analysis of the modification of genes expression. Exploitation of the huge amount of data generated by the microarray screening chip U133 Plus 2.0 is complex (see Effects on keratinocytes), however this technique constitutes one of the best tools to formulate more accurate and comprehensive hypotheses concerning the mechanisms of biological effects of EMF [Collard et al., 2013, 2015; Hinsenkamp et al., 2011, 2015].

See also "Etude des effets d’un courant électrique pulsé de basses fréquences sur les kératinocytes humains" (J.-F. Collard, PhD thesis): http://difusion.ulb.ac.be.

The COST Action BM1309 EMF-MED - European network for innovative uses of EMFs in medical applications. Active implication of our lab:

  • J.-F. Collard is the Belgian Representative on Management Committee on the COST Action. He promotes the interest of the study of cellular mechanisms and importance of the synthesis of actual knowledge.
  • Prof. M. Hinsenkamp is the Belgian Representative substitute on Management Committee on the COST Action.

 

2013-2014 activity report

M. Hinsenkamp, J.-F. Collard

This year has been devoted to the follow up of the activities started during the 2009-2013 project (see the summary below for further information).

Past activity reports

2009-2013

M. Hinsenkamp *1, J.-F. Collard *1, F. Corazza *2, B. Mertens *3,1, V. Cosmin Lazar *4, Y. Le Priol *5, A. Nonclercq *6

*1 Laboratoire de Recherche en Orthopédie Traumatologie; Hôpital Erasme-ULB, Brussels, Belgium
*2 Laboratory of Hematology, Hôpital Brugman-ULB, Brussels, Belgium
*3 Bio-, Electro- And Mechanical Systems Laboratory (BEAM), Faculté des Sciences Appliq, ULB, Brussels, Belgium
*4 Computational Modeling Lab (CoMo), Department of Computer Science, VUB, Brussels, Belgium
*5 Solution Specialist EMEA, Elsevier
*6 Laboratoires d'Images, Signaux et Dispositifs de Télécommunications" (LIST), groupe Traitement des Signaux. Faculté des Sciences Appliquées, ULB, Brussels, Belgium.

The aim of the present research is to demonstrate if electromagnetic fields with a sine wave pattern of 50Hz have a direct or indirect effect on biological process. Starting from biological models showing significant modifications (ea. acceleration of cell differentiation) after exposition to specific low frequencies and low amplitude asymmetric pulses trains modulated electric or electromagnetic fields, we investigate if some characteristics of those patterns and their possible biological effects could be generated by the sinwave 50Hz (1).

We try to identify the biological mechanism involved at the cell level through the microarray screening. For this study, we use keratinocytes cultures harvested from human abdominoplasty. We analyse the genes expressions of 38.500 well-characterized human genes using Affymetrix® microarray chips. The application of ELF by platinum electrodes on keratinocytes cells involves a modification of the genes expression. Some up regulated gene, like DKK1, TXNRD1, ATF3, MME, MACF1(2), BMP-2 plays role in proliferation and differentiation regulation. For exemple, BMP-2 is a key protein in osteoblast differentiation (3, 4).

Our results confirm our previous observations with its negative regulation on the Wnt pathway, the up regulation of the DKK1 expression is also in agreement with a reduction of cell proliferation and an induction of cell differentiation. SAPK/JNK regulated DKK1 expression and is activated by a variety of environmental stresses, inflammatory cytokines and growth factors. An ELF receiver could complete these categories (2).

Other genes, with a later regulation stage, are being analyzed and confirm the results previously obtained.

We published in the international journal Molecular Biology and Biophysics (5) a study, made by a triangular method of analysis, showing that the ELF stimulation accelerates some natural cellular processes. Our results show that ELF stimulation accelerates the up or down-regulation of some genes which in normal circumstances will follow that particular trend (up or down-regulation) but in a slower manner. The genes identifies within this study are involved in cell proliferation and differentiation.

In the most frequent child leukaemia, the Acute Lymphoblastic Leukaemia (ALL), the concentration of ß -catenin and the activation of WNT Signaling Pathway seems increased. In our result we observed a totally opposite reaction: the ß -catenin present in the WNT Signaling Pathway and the WNT Signaling pathway are inhibited with the presence of DKK1 (2).

In all our experimental results we never observed a pathological effect but only acceleration or inhibition of normal physiological process.

See further information on EMF effects on keratinocytes.

(1) Hinsenkamp M, Burny F, Donkerwolcke M, Schuind F, Collard JF. Le Laboratoire de Recherche en Orthopédie Traumatologie – LROT. Revue Médicale de Bruxelles. 32(6 Suppl):S16-22 (2011)

(2)Collard, J.-F., Mertens, B. and Hinsenkamp, M. In vitro study of the effects of ELF electric fields on gene expression in human epidermal cells. Bioelectromagnetics, 32:28-36 (2011).

(3)Hinsenkamp, M. and Collard, J.-F. Bone Morphogenic Protein-mRNA upregulation after exposure to low frequency electric field. Int Orthop, 35(10):1577-81(2011).

(4) Hinsenkamp M. Effets des champs électriques et électromagnétiques sur la différenciation cellulaire et leur intérêt en chirurgie orthopédique et traumatologique. Bulletin et Mémoires de l'Académie Royale de Médecine de Belgique. 166 (7-8-9):307-16 (2011)

(5) Collard, J.-F., Lazar C., Nowé A., Hinsenkamp M. Statistical validation of the acceleration of the differentiation at the expense of the proliferation in human epidermal cells exposed to extremely low frequency electric fields. Progress in Biophysics and Molecular Biology, 111(1):37-45. (2013)

2005-2009

M. Hinsenkamp, JF. Collard, P. Bergman, S. Prohoroff, P. Soularue, A. Bogdan

In vitro Study of the Effects of Electromagnetic Field on Cell Sensitivity (Differentiation/Proliferation)

An acceleration of the differentiation at the expense of the proliferation is observed after the application of a specific low frequency and low amplitude electric and electromagnetic fields on various biological models. At present, we use culture of keratinocytes on a decellularized human dermis on air-liquid interface (very similar to in vivo physiological epidermic development). Two platinum electrodes are used to apply the electric signal. This model of epidermal growth exposed to electric signal provides simplified and well characterized model to study the biological effects of electromagnetic stimulation. The previous results in this model shown on the stimulated cultures a decrease of growth area surrounding the explants; a better stratification with an increased number of cell layers and a decreased percentage of cells marked by [H 3 ]-Thymidine. Those results are also in agreement with an acceleration of the cell differentiation at the expense of the cell proliferation. Following these results showing significant modifications, we try to identify the biological mechanism involved at the cell level through the microarray screening. Microarray technology allows the analysis of the genes expressions of 38500 well-characterized human genes.

The application of ELF by platinum electrodes on keratinocytes cells results in a modification of the genes expression. Some upregulated gene (p=0.05 and 2=Fold Change (FC)=-2), are involved in proliferation and differentiation mechanism. T he up regulation ( FC= 4.42 and p=0.01) of the Dickkopf Homolog 1 (DKK1) expression, with is negative regulation on the Wnt pathway, is also in agreement with a reduction of cell proliferation and an induction of cell differentiation. SAPK/JNK regulated DKK1 expression and is activated by a variety of environmental stresses, inflammatory cytokines and growth factors. An ELF receiver could be add to this list. The Down regulation (FC=-2.66 and p=0.008) of Microtubule-Actin Crosslinking Factor 1 (MACF1) expression plays also a role in Wnt pathway and in the induction of cell differentiation. T hese results were compared with the literature of gene expression in acute lymphoblastic leukaemia which shows a reversed pattern.

See further information on EMF effects on keratinocytes.

2001-2005

M. Hinsenkamp & Z. Batarilo

The aim of present research is to ascertain if electromagnetic fields with a sinwave pattern of 50Hz have a direct or indirect effect on biological processes. Starting from biological models showing significant modifications (eg. acceleration of cell differentiation) after exposure to specific low frequencies and low amplitude modulated and, of electrical or electromagnetic nature, we investigate to determine whether some characteristics of those patterns and their possible biological effects could be generated by the sinwave 50Hz.

Our strategy includes three different axes of research, which could allow the identification, at their cross junction, of possible receptors and biological mechanisms:

  • Description and characterization of the tissular response to a specific EM field.
  • Identification of the cellular mechanism triggered by the EM signal and investigation of a "cellular receptor mechanism".
  • Classification of the physical characteristics of the EM fields.

A series of genes playing a part in the differentiation and the cellular proliferation, a part in the "protection" of the cells or the synthesis of proteins, could be highlighted. Molecules like the MAPK, the Katanines, the Integrines, the Transglutaminases will have to be monitored in our next research with a new Affymetrix chip of 38,500 genes.

Our studies have shown that the modification of some characteritics of the electromagnetic fields have a significant effect on biological response. Three-dimensional analysis of potential and current distribution of each electrical pattern can be used to correlate the exact values with biological response as well as to reproduce the same biological effects with different types of stimuli by comparing the results of 3D simulation. This analysis could be applied to other biological experiments and accelerate the synergy of various teams of the BBEMG group by comparing precise electric field characteristics, which appears inside the biological model.

See further information on EMF effects on keratinocytes.
See further information on EMF effects on bones.

1999-2000

M. Hinsenkamp

We are currently using an experimental model to study the behaviour of human skin cells. We observe the same acceleration of cell differentiation exposed to a complex electric signal. Modifications of some characteristics of this specific signal produce different results on cell growth and differentiation. Recent results confirm the effects of complex electromagnetic fields on cell differentiation by an increase of Growth Factors.

Using the same biological model, we have begun to study the expression of messenger RNA to articulate the cell mechanism involved in transcription of the electromagnetic signal. Preliminary results are promising.

See further information on EMF effects on keratinocytes.
See further information on EMF effects on bones.

Publications

Publications related to BBEMG activities

Books

Hinsenkamp M.
Contribution à l'étude des facteurs physiques influençant la consolidation osseuse.
Mémoire ayant reçu le Prix du Concours Ordinaire 1994-1995 de l'Académie Royale de Médecine de Belgique, 86 pages, 1995.

Hinsenkamp M., Rooze M.
Current concepts in bioelectromagnetics.
Elsevier Sequoia, Lausanne, 345 pages, 1993.

Hinsenkamp M., Burny F.
Electromagnetic stimulation of bone growth and repair.
Munksgaard, Copenhagen, 92 pages, 1982.

Andersen J.B., Borrell J., Chiabrera A., D'inzeo G., Hinsenkamp M., Leal J., Miro L., Nieto Sampedro M., Rozzell T., Veyret B.
The European bioelectromagnetic center.
Spanish Ministry of Health, Servicio Editoriales, Madrid, 212 pages, 1993.

Hinsenkamp M.
Stimulation électromagnétique de l'ostéogénèse et de la consolidation des fractures.
Académie Royale de Belgique, Classe des Sciences, Bruxelles, 336 pages, 1993.

Burny F., Herbst E., Hinsenkamp M.
Electric stimulation of bone growth and repair.
Springer Verlag, Berlin, Heidelberg, New-York, 94 pages, 1978.

Articles published in science journals

Collard JF, Hinsenkamp M.
Cellular processes involved in human epidermal cells exposed to extremely low frequency electric fields.
Cellular Signalling, 27(5):889-898, 2015.
http://www.ncbi.nlm.nih.gov/pubmed/25683910

Collard J.-F., Lazar,C., Nowé A., & Hinsenkamp M.
Statistical validation of the acceleration of the differentiation at the expense of the proliferation in human epidermal cells exposed to extremely low frequency electric fields.
Progress in Biophysics and Molecular Biology, 111(1):37-45, 2013.
http://dx.doi.org/10.1016/j.pbiomolbio.2012.12.004

Hinsenkamp M.
Effets des champs électromagnétiques sur la différenciation cellulaire et son intérêt en chirurgie orthopédique et traumatologique.
Bulletin et Mémoires de l'Académie Royale de Médecine de Belgique. 166 (7-8-9):307-16, 2011.

Hinsenkamp M., Burny F., Donkerwolcke1 M., Schuind F., Collard J.F.
Le Laboratoire de Recherche en Orthopédie Traumatologie – LROT
Rev. Med. Brux., 32(S6):S16-S22, 2011.

Hinsenkamp M, Collard JF.
Bone Morphogenic Protein--mRNA upregulation after exposure to low frequency electric field.
Int Orthop, 35(10):1577-81, 2011.

Collard, J.-F., Mertens, B. & Hinsenkamp, M.
In vitro study of the effects of ELF electric fields on gene expression in human epidermal cells.
Bioelectromagnetics, 32:28-36, 2011.

Hinsenkamp M.
Les Champs électromagnétiques.
In : « La science face au défi du paranormal », (Ed. J. Dommanget), Quorum, Gerpinnes : 163-169, 1999.

Hinsenkamp M., Jercinovic A., Heenen M., De Graef Ch., Wilaert Ch.
Effects of alternating electrical current on keratinocytes in vitro (AC).
Bioelectromagnetics, 18: 250-254, 1997.

Jercinovic A., Hinsenkamp M., Wilaert F., De graef Ch., Heenen M., Goldschmidt D.
Effects of direct constant current on keratinocytes in vitro (DC).
Bioelectroch. Bioener., 39 (2): 209-214, 1996.

Hinsenkamp M.
Influence des facteurs physiques sur la consolidation osseuse.
Bulletin et mémoires de l'Académie Royale de Médecine de Belgique, 151 (12): 517-526, 1996.

Hinsenkamp M.
15 Years Experience in electromagnetic stimulation of bone growth and repair.
J. Jpn. Bioelect. Res. Soc., 8: 1-10, 1994.

Hinsenkamp M.
Clinical studies of the effects of electromagnetic fields on bone tissues.
Cost 244: Biomedical effects of electromagnetic fields. (Ed.: Simunic D.) EU. DG XII: 114-119, 1994.

Hinsenkamp M., Hauzeur J.P., Sintzoff S. Jr.
Preliminary results in electromagnetic field treatment of osteonecrosis.
Bioelectroch. Bioener., 30: 229-235, 1993.

Hinsenkamp M., Heenen M., Dierickx M., Lifschitz L.
Effects of electrical stimulation on wound healing.
Final report contract EC n ° CI1-0348-B, complementary to: CI1-0349-YU: pp. 46, 1993.

Tavernier A., Dierickx M., Hinsenkamp M.
Tensors of dielectric permittivity and conductivity of in vitro human derms and epiderms.
Bioelectroch. Bioener., 30: 65-72, 1993.

Debouck c., Rooze m., Hinsenkamp M.
Effect of electromagnetic field amplitudes on quail embryos: preliminary results.
Bioelectroch. Bioener., 30: 189-193, 1993.

Hinsenkamp M., Hauzeur J.P., Sintzoff S.
Results in electromagnetic fields (EMF) treatment of osteonecrosis.
In: "Bone Circulation and Vascularization in normal and pathological conditions".
Eds: A. Schoutens, J. Arlet, J. Gardeniers, S. Hughes, Plenum: 331-336, 1993.

Andersen B., Borrell J., Chiabrera A., D'inzeo G., Hinsenkamp M., Leal J., Miro J., Nieto Sampedro M., Rozzel T., Veyret B., Bersani F., Rooze M., Semm P.
The European Bioelectromagnetic Center.
Spanish Ministry of Health, Servicio Editoriales, Madrid: pp. 212, 1993.

Hinsenkamp M.
Stimulation électromagnétique de la croissance et de la consolidation osseuse.
Thèse d'Agrégé de l'Enseignement Supérieur de l'U.L.B.: pp. 381, 1990.

Hinsenkamp M., Rooze M.
Traitements par champs électromagnétiques pulsés: indications et limites.
In: "Enseignement Postuniversitaire 1986", Pub. U.L.B.: 105-111, 1986.

Hinsenkamp M., Lheureux Ph., Martins D.
Transmembrane Na/K exchanges under electromagnetic fields. Preliminary study on human erythrocytes.
Reconstr. Surg. Traumat., 19: 63-69, 1985.

Hinsenkamp M., Rooze M., Noorbergen M., Tuerlinckx B., Coussaert E.
Topography of EM exposure and its relationship to biological effects on tissues.
In: "Interactions between electromagnetic fields and cells", (Eds.: Chiabrera A., Nicolini C. and Schwan H.), Plenum Press: 557-567, 1985.

Hinsenkamp M., Ryaby J., Burny F.
Treatment of non-union by pulsing electromagnetic field: European multicenter study of 308 cases.
Reconstr. Surg. Traumat., 19: 147-151, 1985.

Hinsenkamp M., Schoutens A.
Evolution of radionuclide scintimetry of non-unions treated by electromagnetic stimulation. Preliminary study.
Reconstr. Surg. Traumat., 19: 113-117, 1985.

Hinsenkamp M., Tuerlinckx B., Rooze M.
Effect of ELF fields on bone growth and fracture repair.
In: "Biological effects and dosimetry of static and ELF electromagnetic fields", (Eds.: Grandolfo M., Michaelson S. and Rindi A.), Plenum Press: 441-476, 1985.

Rooze M., Hinsenkamp M.
In vivo modifications induced by electromagnetic stimulation of chicken embryos.
Reconstr. Surg. Traumat., 19: 87-92, 1985.

Tuerlinckx B., Boido M., Hinsenkamp M.
Analysis of the electromagnetic fields caused by bone growth stimulators. A boundary-element simulation program.
Reconstr. Surg. Traumat., 19: 3-18, 1985.

Hinsenkamp M.
Electromechanical hypothesis of bone demineralization in weightlessness.
In: "The Gravity Relevance of Bone Mineralisation Processes". ESA.SP-203: 53-58, 1984.

Hinsenkamp M., Burny F., Donkerwolcke M., Coussaert E.
Electromagnetic stimulation of fresh fractures treated with Hoffmann external fixation.
Orthopedics 7: 411-416, 1984.

Hinsenkamp M.
Die Behandlung von Falschgelenkbildung mit der elektromagnetischen Stimulation.
Extracta Orthop. 6: 13-29, 1983.

Hinsenkamp M.
Mechanical mediator of bone demineralization in weightlessness: a biolectromagnetic hypothesis.
In: "Space Physiology", CEPADUES Ed.: 247-253, 1983.

Hinsenkamp M., Rooze M.
Morphological effect of electromagnetic stimulation on limb skeleton of the new born mouse.
Acta Orthop. Scand., Suppl. 196: 39-50, 1982.

Dierickx M., Hinsenkamp M., Rybowski L., Burny F.
Electromagnetic and electric field configuration produced by two coils.
Acta Orthop. Scand., Suppl. 196: 19-38, 1982.

Rooze M., Hinsenkamp M.
"In vitro" histochemical modifications induced by electromagnetic stimulation.
Acta Orthop. Scand., Suppl. 196: 51-62, 1982.

Pegoff S., Georges B., Rybowski L., Dierickx M., Hinsenkamp M., Burny F.
Application of the Z transform to biological impedance measurements.
Acta Orthop. Scand., Suppl. 196: 15-18, 1982.

Georges B., Pegoff S., Rybowkski L., Wynan R., Fontana A., Dierickx M., Hinsenkamp M., Burny F.
Electrochemical study of electrodes behavior during bioelectric impedance measurements.
Acta Orthop. Scand., Suppl. 196: 9-14, 1982.

Chiabrera A., Hinsenkamp M., Pilla A., Nicolini C.
Electrochemical information transfer from cell surface to chromatin under electromagnetic exposure.
In: "Chromatin structure and function" part B, (Ed.: Nicolini C.), Plenum Press, NATO Advanced Study Institutes Series, Serie A, 21: 811-840, 1979.

Chiabrera A., Hinsenkamp M., Pilla A., Ryaby J., Ponta D., Belmont A., Beltrame F., Grattarola M., Nicolini C.
Cytofluorometry of electromagnetically controled cell dedifferentiation.
J. Histochem. Cytochem., 27: 375-381, 1979.

Rooze M., Hinsenkamp M.
Effet de la Stimulation électromagnétique sur l'ostéogénèse.
Acta Orthop. Belg., 45, 587-594, 1979.

Hinsenkamp M., Bourgois R., Bassett C.A.L., Chiabrera A.,Burny F., Ryaby J.
Electromagnetic stimulation of fracture repair. Influence on healing of fresh fractures.
Acta Orthop. Belg., 44: 671-698, 1978.

Hinsenkamp M., Burny F., Donkerwolcke M., Dierickx M.
Modifications of electric potentials of the pins of Hoffmann's "fixateur externe" during fracture healing.
Acta Orthop. Belg., 44: 732-737, 1978.

Hinsenkamp M., Burny F., Jedwab J., Bourgois R.
Corrosion of implants during electric stimulation of fracture healing.
In: "Stimulation of Bone Growth and Repair", Eds. Burny F., Herbst E. and Hinsenkamp M. Eds., Springer Verlag, Heidelberg: 77-83, 1978.

Hinsenkamp M., Chiabrera A., Ryaby J., Pilla A., Bassett C.A.L.
Cell behaviour and DNA modification in pulsing electromagnetic fields.
Acta Orthop. Belg., 44: 636-650, 1978.

Wollast R., Hinsenkamp M., Burny F.
Physicochemical effect of an electric potential on bone growth.
In: "Stimulation of Bone Growth and Repair", Eds. Burny F., Herbst E. and Hinsenkamp M., Springer Verlag, Heidelberg: 29-33, 1978.

de Fraiteur M., Dubois T., Hinsenkamp M., Rybowski L., Dierickx M., Donkerwolcke M., Burny F.
Impedance of living tissues. Proposal for equivalent circuits.
Acta Orthop. Belg., 44: 651-658, 1978.

Dierickx M., Rybowski L., Hinsenkamp M., Burny F.
Electromagnetic Phenomena.
Acta Orthop. Belg., 44: 615-635, 1978.

Other publication fields

 Hinsenkamp M., Collard JF.
Growth factors in orthopaedic surgery: demineralized bone matrix versus recombinant bone morphogenetic proteins.
International Orthopaedics, 39(1):137-47, 2015.

Hinsenkamp M., Mathieu F., Claus W., Collard JF., de Maertelaere V.
Effects of physical environment on the evolution of Kashin-Beck disease in Tibet.
International Orthopaedics, 33(5): 1085-1088, 2009.

Hinsenkamp M., Burny F.
Hommage à Robert de Marneffe (1919-2007).
Rev. Chir. Orthop., 95(1): 92-93, 2009.

Malaisse F., Lognay G., Haubruge E., De Kessel A., Delcarte E.,
Wathelet B., Van Damme P., Begaux F., Chasseur C., Drolkar P.,
Goyens P., Hinsenkamp M., Leteinturier B., Mathieu F., Rinchen L., Van Marsenille C., Wangdu L., Wangla R.
The alternative food path or the very little diversified diet hypotheses.
In: "Big Bone Disease", (Eds. Malaisse F., Mathieu F.), Presse Agro de Gembloux, pp. 105-130, 2008.

Mathieu F., Rinchen L., Wangdu L., Begaux F., Suetens C., Demartelaer V., Hinsenkamp M.
Physical Therapy Study.
In: "Big Bone Disease", (Eds. Malaisse F., Mathieu F.), Presse Agro de Gembloux, pp. 61-63, 2008.

Hinsenkamp M., Mathieu F., Begaux F., Sandrup T., Stallenberg B.
Radiological Study.
In: "Big Bone Disease", (Eds. Malaisse F., Mathieu F.), Presse Agro de Gembloux, pp. 57-59, 2008.

Mathieu F., Suetens C., Begaux F., Rinchen L., Wangdu L., De Martelaer V., Hinsenkamp M.
Clinical Study.
In: "Big Bone Disease", (Eds. Malaisse F., Mathieu F.), Presse Agro de Gembloux, pp. 51-56, 2008.

Mathieu F., Hinsenkamp M.
Kashin-Beck Disease.
In: "Big Bone Disease", (Eds. Malaisse F., Mathieu F.), Presse Agro de Gembloux, pp. 11-18, 2008.

Guerroudj M., de Longueville J.C., Rooze M., Hinsenkamp M., Feipel V., Schuind F.
Biomechanical Properties of Triceps Brachii Tendon after In Vitro stimulation of different posterior surgical approaches.
J. Shoulder Elbow Surg., 16(6): 849-853, 2007.

Hinsenkamp M.
Introduction
In: "75th Anniversary Book of SICOT" (Ed: Sorbie C.), 9-11, 2005.

Baillon R., Batarilo Z., Hinsenkamp M., Rooze M.
Validation d'une méthode de mesure de l'enfoncement d'un implant fémoral cimenté.
Rev. Chir. Orthop., 90(3): 232-240, 2004.

Gangji V., Hauzeur J.P., Schoutens A., Hinsenkamp M., Appelboom Th., Egrise D.
Abnormalities in the Replicative Capacity of Osteoblastic Cells in the Proximal Femur of Patients with Osteonecrosis of the Femoral Head.
J. Rheumatol, 30: 348-351, 2003.

Egrise D., Holy X., Hinsenkamp M., Begot L., Schoutens A., Bergmann , Zerath E.
Protracted systemic changes in bone biology after segmented unloading in the rat .
Calcified Tissue Int., 73(1), 56-65, 2003.

Hinsenkamp M., Ryppens F., Begaux F. et al
The Anatomical Distribution of Radiological Abnormalities in Kashin-Beck Disease in Tibet.
In: Year Book of Diagnostic Radiology 2002 (Ed. Anne G. Osborn), Mosby: 185-187, 2002.

Pasteels J.L., Liu Fu-De, Hinsenkamp M., Rooze M., Mathieu F., Perlmutter N.
Histology of Kashin-Beck Lesions.
International Orthopaedics, 25(3): 151-153, 2001.

Mathieu F., Begaux F., Suetens C., De Martelaer V., Hinsenkamp M.
Anthropometry and clinical features of Kashin-Beck disease in central Tibet
International Orthopaedics, 25(3): 138-141, 2001.

Mathieu F., Suetens C., Begaux F., De Martelaer V., Hinsenkamp M.
Effects of physical therapy for patients suffering from Kashin-Beck disease in Tibet.
International Orthopaedics, 25(3): 191-193, 2001.

Hinsenkamp M,. Rippens F., Begaux F., Mathieu F., De Maertelaer V., Lepeire M., Rooze M., Haubruge E., Chasseur C., Stallenberg B.
Anatomic distribution of radiographic abnormalities in Kashin-Beck disease.
International Orthopaedics, 25(3): 142-146, 2001.

Haubruge E., Chasseur C., Debouck C., Begaux F., Suetens C., Mathieu F., Michel V., Gaspar C., Rooze M., Hinsenkamp M., Gillet P., Nolard N., Lognay G.
Mycotoxins prevalence in KBD.
International Orthopaedics, 25(3): 159-161, 2001.

Hinsenkamp M.
Editorial: Kashin-Beck Disease.
International Orthopaedics, 25(3): 133, 2001.

Vico L., Hinsenkamp M., Jones D., Marie P., Zallone A., Cancedda R.
Osteobiology, strain and microgravity. Part II: studies at the tissue level.
Calcified Tissue Int., 68(1): 1-10, 2001.

Hinsenkamp M., de Longueville J.C., Burny F.
Bone formation and resorption studies in microgravity.
In: Microgravity I, (Eds. OSTC): 143-149, 2001.

Hinsenkamp M.
Kashin-Beck disease.
Guest Editorial in : « Orthopaedics Today», 3(6) : 3-6, 2000.

Marie P., Jones D., Vico L., Zallone A., Hinsenkamp M., Cancedda R.
Osteobiology, strain and microgravity. Part I: Studies at the cellular level.
Calcified Tissue Int., 67(1) : 2-9, 2000.

Liu F., Wang Z., Hinsenkamp M.
Knee osteotomy in 195 advanced cases of Kashin-Beck disease.
Int. Orthop., 22: 87-91, 1998.

Panda M., Ntungila N., Kalunda M., Hinsenkamp M.
Treatment of chronic osteomyelitis using the Papineau technique.
Int. Orthop., 22: 37-40, 1998.

Additional information

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